Sentence examples for by a rapid application from inspiring English sources
SR Ca2+ load was estimated by a rapid application of 10 mM caffeine via a nearby pipette.
Mobilization typically involves a slow and sustained application of iatrogenic force, while manipulation is characterized by a rapid application of iatrogenic force.
SR Ca content was quantitatively measured by a rapid application of 10 mM caffeine and integration of the resulting inward NCX current.
Furthermore, a rapid application of the calcium ionophore, A23187, caused the fluorescence to greatly exceed that produced by glutamatergic stimuli (Supplementary Fig. S4; Supplementary Movies 4 and 5).
SR Ca load was evaluated by Ca transient amplitudes induced by rapid application of caffeine (10 mmol/L).
In spite of this 50-fold increase in ACh application rates, synchrony reemerged, escaping the clamp, with Sync Ratio values partially decreasing again only after substantial ACh buildup, caused by the rapid application rates.
To confirm this, the SR load was probed by rapid application of 10 mM caffeine, following a 10 s suspension of pacing.
SR Ca content was measured by rapid application of 10 mM caffeine and integration of the resulting inward NCX current as described previously in detail [11].
Fig. 2A (representative of 5 cells) shows specimen NCX currents (top) produced by rapid application of 10 mM caffeine in control and following TBQ and the integrals of those currents (below).
The SR Ca2+ content was assessed by rapid application of 10 mM caffeine [within ∼1 s of the end of the 90 s voltage-pulse train, using a 4 mm diameter perfusion pen (Cell Micro Controls, Norfolk, USA) in close proximity to the cell] to fully release SR-Ca2+ stores, and estimated as the absolute time-integral of the resultant INa/Ca, expressed in coulombs normalized to capacitance.
This notion is supported by the inhibitory effect of rapid application of a low [Na+]e solution at physiological (this study) and ambient (reported previously [13]) temperatures.
