Sentence examples for by a random primer from inspiring English sources

Briefly, 100 ng of genomic DNA were labeled by a random primer reaction during two hours.

One half μg of digested and ethanol-purified DNA and normal female reference DNA were labeled by a random primer reaction with Cy3-dUTP and Cy5-dUTP (Amersham Pharmacia Biotech Inch)., respectively, and co-hybridized to the array slides in 48 hours at 37°C by use of an automated hybridization station (GeneTAC, Genomic Solutions/Perkin Elmer) and our established protocol [ 50].

Each cDNA probe was purified by agarose gel electrophoresis, recovered by using a QIAEX gel extraction kit (QIAGEN Inc., Chatsworth, CA, USA), and radiolabelled by a random primer technique with a commercially available kit (Amersham Life Science Inc., Arlington Heights, IL, USA).

In brief, TAP treatment was followed by ligation with an excess of 5′ adapter (Illumina TruSeq Small RNA kit) and by reverse transcription using a random primer (RPO primer: 5′CCTTGGCACCCGAGAATTCCANNNNNN-3′).

Oligonucleotide 1 and 2 were also used to generate non-radioactive and radioactive probes by utilizing the PCR DIG Probe Synthesis Kit from Roche Diagnostics (Mannheim, Germany) or by α-P]dNTP incorporation using a Random Primers Labeling System (Gibco-BRL, Cergy-Pontoise, France), according to the supplier's protocol.

Then the first strand cDNA was synthesized by a random hexamer-primer using the mRNA fragments as templates.

The membrane was analysed using indirect end-labelling with a probe generated by random primer labelling from a 151 bp PCR product prepared using the primers 5'-tgtgcaaattccaactaaagga-3' and 5'-ggcgataatttatcatgttttcc-3'.

IS CR and sul2 PCR product amplified with primers CRF/CRFF-r were labeled with P-ATP by random primer extension by using a commercially available kit (Stratagene, Amsterdam, the Netherlands) according to the manufacturer's instructions.

The cRNA (3 μg) was reverse transcribed in the first-strand cDNA synthesis step by using a random hexamer primer, followed by RNase H-mediated second-strand cDNA synthesis in replicates.

With this technique, target RNAs of ten potato viruses were reversal transcribed by random primer in a single reaction, then subjected to LDR and asymmetric labeling PCR as template, finally, the MRLP amplicons were analyzed by microarray hybridization and subsequent scanning.

In brief, 2 µg of genomic DNA from test sample (GK) and 2 µg from reference (Wistar) were digested by AluI/RsaI and labeled by random primer, incorporating Cy5 (red) and Cy3 (green) fluorescent dyes.