Sentence examples for by a purification step from inspiring English sources

The analytical procedure is based on solvent extraction followed by a purification step with graphitized cartridges and CYN quantification by LC MS/MS.

Protein labelling with gallium-68 is currently not straightforward, because currently available bifunctional chelators typically require relatively harsh labelling conditions such as high temperature, low pH and high chelator-conjugate concentration, often followed by a purification step [18 25].

We performed a preliminary extraction step [31], [32] followed by a purification step using immunoaffinity chromatography columns and reverse-phase high performance liquid chromatography prior to the testosterone radioimmunoassay.

Method C: TRIzol RNA isolation (Invitrogen) followed by a purification step (RNeasy Mini Kit, Qiagen).

First, a regular PCR was performed followed by a purification step with the QIAquick PCR Purification Kit (250) (Germany, QIAGEN).

DNA (1 μg) was treated with sodium bisulfite followed by a purification step using the EZ-96 DNA Methylation-Gold kit (Zymo Research, Orange, CA, USA).

Remaining traces of genomic DNA were removed by DNase after a purification step with the CleanUp protocol of the RNeasy Plant Mini kit (Qiagen).

Besides, not only a higher atomic yield is achieved, with respect to the conventional biodiesel reaction (because no glycerol by-product is generated), but also a purification step of residual glycerol is not necessary, so it can be used directly after its production.

Briefly, urine samples, after deconjugation with helix pomatia, were subjected to a purification step by solid phase extraction (SPE) on a C18 column (500 mL/6 mL) followed by a filtration through a NH2 SPE cartridge (100 mg, 1 mL), while liver samples were extracted with acetonitrile and purified by a single C18 (500 mL/6 mL) SPE step.

For oestradiol (and free oestradiol), oestrone, androstenedione and testosterone (and free testosterone), the results were examined according to whether or not the hormone assay had included a purification step (usually by organic solvent extraction and column chromatography).

(A) Purification steps followed by SDS-PAGE.