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Xenopus laevis primary hepatocytes were cultured by a protocol adapted from a published report [ 55].
Agroinoculation of T200 and TME3 cassava plantlets was achieved by a protocol adapted from Hayes et al. [ 153].
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DNA was extracted by using a protocol adapted from Lamour and Finley (27 ).
Catalase (CAT) activity was measured by using a protocol adapted from [ 29].
DNA was transfected into cells by electroporation using a protocol adapted from Maniatis.
The complex was then extracted by cyclohexane according to a protocol adapted from [ 72].
Clontech's SMART cDNA Library Construction kit (Clontech, USA) was used for cDNA synthesis with a protocol adapted by K.V. Donohue (personal communication), and provided by the Genomic Sciences Laboratory, North Carolina State University.
Using a protocol adapted by clinic policy fellow Talia Sechley MEM '16 to suit smaller, inland cities, students put on waterproof boots and sampled litter from streams around Durham and Duke, cataloguing the types and locations of their finds.
In addition, when separated by reversed-phase HPLC using a protocol adapted for defensin isolation, the 40% Sep-Pak-fractionated plasma from bacteria-challenged larvae displayed dissociated antimicrobial activity and anti-Spod-11-tox immunoreactivity.
Microscopic preparations were performed according to a protocol adapted by Gognies et al. [ 50].
Male prairie vole fibroblast cell cultures were established from a tissue homogenate produced by manual and enzymatic digestion using a protocol adapted from [ 26].
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