Your English writing platform
Discover LudwigSuggestions(5)
Exact(1)
Importantly, the observed lack of 1-indanone conversion is not caused by a poor expression because all PAMO variants were equally well expressed under these experimental conditions (data not shown), thereby pointing towards a low reactivity of PAMO P440L and P440G with 1-indanone like the wild-type enzyme and possibly a lowered uptake of the substrate under non-optimized conditions.
Similar(59)
When cells have not yet established well-defined adherens junctions, as shown by the poor expression of E-cadherin at cell cell contacts (left panel), PrPc was mainly detected intracellularly.
The large-scale production of recombinant pharmaceutical proteins is often hampered by the poor expression of their mature, active forms in prokaryotic hosts such as E. coli and by the high costs and the limited scalability of traditional fermenter-based platforms using mammalian cells.
This issue is further complicated by poor expression of HIV structural antigens due to rev dependence.
However, different dual-gene expression vector strategies are limited by poor expression of one or both transgenes.
In particular, the liver sections of group 3) were characterized by poor expression of VEGF in endothelial cells that located in the wall of the vena centralis and sinusoids.
This is probably caused by a poor induction of PAMO expression at 0.2% arabinose as BL21 DE3) is able to metabolize arabinose, which, may, therefore, impair induction of PAMO production.
This is in agreement with the poor expression observed by immunofluorescence.
We conclude that expression of correctly folded and targeted aquaporins is strongly dependent on recombinant gene dosage and also that increasing the gene dosage could, to some extent, help improving poor expression caused by inefficiency at the transcriptional and translational levels.
The absence of obvious autoimmune lesions may be due to poor expression of HER-2 by adult hamster tissues.
The loss of IL-15 also resulted in poor expression of key effector molecules such as IFN-γ, granzyme A and C, as well as several NK cell receptors, which are also regulated by T-bet in NKT cells.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com