Sentence examples for by a plasmid vector from inspiring English sources

After being harvested and characterized in vitro, BM-MSCs were infected by a plasmid vector containing BMP-7 encoding gene enriched with a heparin-binding site (B2BMP-7) to assess its osteogenic effects in vivo.

According to this protocol, hGFs were first infected with a lentivirus to express the mouse Slc7a1 gene (by a plasmid vector from Addgene) and then infected with retroviruses coding human c-MYC, OCT3/4, SOX2 and KLF4 genes for iPS cell induction.

gRNAs are introduced to cells by a plasmid vector, either through transfection or viral infection.

To investigate whether Tcl1 is a major mediator of the effects of Oct3/4 on the proliferation of ES cells, we established multiple stable cell clones (Tcl1- 2A1, Tcl1- 2D5, and Tcl1- 1A6) by transfecting a plasmid vector constitutively expressing Tcl1 into ZHBTc4 ES cells.

Quantitative real-time PCR (qPCR) was used to assess differences in the steady state mRNA levels of genes in response to the proteins of interest by comparison to a plasmid vector treated control.

As anti-Ad antibodies resulting from the initial immunization of the mice can potentially interfere with the efficacy of the rAd vector to function as a vaccine carrier during booster immunizations, we adopted a heterologous prime/boost strategy that entailed priming the animals with the rAd vector, followed by boosting with a plasmid vector encoding the same bivalent antigen gene.

The synthetic controls were amplified by PCR, cloned into a plasmid vector and transferred into bacteria host cells.

To determine if the BMPR1B-BMPR2 recomplexcomplex is essential for the effects of GDF5 on dendrite growth, we manipulated the function of these receptors in hippocampal neurons by transfecting them with a plasmid vector that expresses both GFP and a mutated BMP receptor protein.

The aim of the present study was to establish a method for gene transfer into the periodontal ligament (PDL) by in vivo electroporation with a plasmid vector and to investigate the effects of BMP-4 transfer into the PDL.

Viral copy number was calculated by standard curve analysis using a plasmid vector containing the EBOV amplicon.

cDNA corresponding to the complete sequence of 7 of 10 double-stranded RNA segments of the viral genome was amplified by PCR and cloned into a plasmid vector.