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Each object was presented for 30 seconds haptically or 15 seconds visually; these times were determined by a pilot experiment.
The range of target densities depended on both test conditions and noise density and was determined by a pilot experiment (data not shown) in which one of the authors served as an observer.
We confirmed the assumption that 2 h at 45 °C are superior to 1 h or 2 h at 50 °C by a pilot experiment (data not shown).
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The fact that amino acids encoded by RWY have simple lateral chains and were synthesized by Miller 31 in a pilot experiment for primordial earth conditions suggests that RWY is a relic of the prebiotic times at the origin of life.
To provide a comparison between the microarray assays and another commonly used measure of gene expression differences, qRT-PCR, gene expression differences (infected versus mock) for a set of selected genes were measured by qRT-PCR during a pilot experiment.
Before conducting our full screen we performed a pilot experiment by transfecting myocytes with BNP-Luc and d-siRNA targeting the CaV1.2 gene or the bacterial gene LacZ.
We therefore could perform a pilot experiment by recruiting a small number of individuals.
To translate our findings to clinical relevance, we conducted a pilot experiment by recruiting human subjects to our studies.
To test for a possible risk of cross-contamination during sample transfer by the STS, we ran a pilot experiment with alternating blank samples and samples containing HPV16-positive SiHa cells and found no evidence of cross-contamination.
In order to identify probes that would perform well for mapping to the D genome, we conducted a pilot experiment by screening probes on a 3*720 K CGH array using genomic DNAs from the hexaploid Chinese Spring wheat (AABBDD) and from the tetraploid durum wheat (AABB) missing the D genome.
Stimuli were presented by showing five cartoons selected from a pilot experiment.
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