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The UV detection of the toxins was performed by a photodiode array detector.
The mobile phase was a mixture of n-Hexane/isopropanolthanol/diethyl amine (850 75:75:0.1, v/v/v/v) with 0.8 mL/min of flow rate, and detection was performed by a photodiode array detector (PDA) at 220 nm.
Ramping was performed as follows: equilibration with 90%% A for 2 min before injection and afterwards a change from 90%to10%0% eluent A in 25 min, followed by holding for 10 min and then returning to 90% eluent A within 10 min. CoA-thioesters were detected at 259 nm by a photodiode array detector.
Boswellic acids were detected by a photodiode array detector, scanning from 190 to 600 nm; and quantification was performed at 205 nm.
MPP+ was detected by a photodiode array detector set at 295 nm, and a triple quadrupole mass spectrometry with a mass to charge ratio of 170 128 at 32 V and 1.9 mTorr (ThermoSurveyor PDA/TSQ Quantum, ThermoScientific, Loughborough, UK).
MPP+ was detected by a photodiode array detector set to 295 nm, and a triple quadrupole mass spectrometre with a mass to charge ratio of 170 128 at 32 V and 1.9 mTorr (ThermoSurveyor PDA/TSQ Quantum, ThermoScientific, Loughborough, UK).
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We continuously monitored absorbance of the column eluate by using a photodiode array detector (Waters 996) to measure hypoxanthine at 254 nm and uric acid at 293 nm.
The determination of tetrandrine from FJHQT extract was carried out by HPLC with a photodiode array detector.
The chromatograms were monitored by UV absorption using a photodiode array detector MD-20188, JASCorp.rp., Tokyo, Japan) and by fluorescence at excitation and emission wavelengths of 460 and 520 nm, respectively, using a fluorescence detector (FP-920, JASCO Corp., Tokyo, Japan).
The reflected light is collected and focused onto a photodiode array by an optical system.
The identification of the constituents of R. tinctorum is carried out by liquid chromatography coupled with a photodiode array detector (LC PDA).
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