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This was implemented by a permutation re-sampling test in IDENTIX version 1.1 (Belkhir et al. 2002).

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By Single Protein Analysis of Net-works SPAN [ 12]) aNet-works SPANe permutation re-sampling, a small, but more biological significant breast cancer signature consisted by 54 genes is identified from a protein interaction network include 250 cancer-related genes curated from literatures [ 12] 16].

We used the permutation re-sampling method [ 47],[ 48] of the original data to model the null distribution.

At each permutation re-sampling, SEP of testing patients was calculated with top-ranked genes obtained from training patients; the mean of accuracy and AUC was weighted by size of testing subgroups; values presented were the medians summarized from10,000 re-samplings.

We use permutation re-sampling of the original data to model the null distribution and calculate the p-value of each pathway edge (see Methods).

Denote the OR, RR and Chi statistic from permutation or re-sampling as x1, x2,… x B where B is the number of permutation or resampling.

To use Fieldtrip's permutation random re-sampling procedures to control FWER, it was necessary that the pre- and post-intervals had the same number of time points.

Using a stringent re-sampling and permutation methodology we were able to show that indeed multiple signatures can be identified showing similar prognostic power while the genes from these different samplings have similar functions.

To eliminate the possibility that the distribution pattern of correlation coefficients is an artifact introduced by the relatively small value of EST counts, we performed a re-sampling procedure and a permutation procedure.

However, the bootstrap threshold algorithm differs from the empirical permutation threshold algorithm as the former generates a new data set based on a bootstrap re-sampling of the centered residual effects.

This permutation consists of re-sampling genes according to the number of genes represented in our dataset per pathway with their P values.

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