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Histologic sections of the primary tumor were reviewed by a pathologist without any clinical data and scored according to the percentage of each cellular component (tumor cells, normal stroma, inflammatory cells, necrosis, fibrosis, and other).
Tissue microarray slides were analyzed by a pathologist without knowledge of clinicopathological parameters of the tumors.
As part of the histological evaluation, all slides were examined by a pathologist, without knowledge of the previous treatment, under a light microscope.
The sections were examined by a pathologist without knowledge of the experimental groups for presence of any negative features, such as edema, erosion, ulceration and necrosis.
Sections were stained with hematoxylin-eosin before they were examined by a pathologist without previous knowledge of the details of the rabbit ileal loop experiments.
As a part of the histologic evaluation, all slides were examined by a pathologist without knowledge of the previous treatment, by using masked slides from ×5 to ×40 magnification with a Leica (Leica Microsystems, Milan, Italy) microscope.
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All immunohistochemistry scoring was performed by a research associate and a pathologist without knowledge of pathological and clinical data.
A pathologist without knowledge of the group identity examined the sections by light microscopy.
Semiquantitative assessment of staining was performed by a pathologist (MA) without prior knowledge of clinical information.
Pathological slides were reviewed by a pathologist (PG) without knowledge of the clinical outcome.
The immunohistochemical analysis was evaluated by a pathologist (RB) without knowledge of the clinicopathological information.
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