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The calculation was automatically processed on the basis of the open reading frame identified by a pairwise alignment between a translated nucleotide sequence and amino acid sequences of orthologs using BLASTX [ 43].
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They were positioned on the E. coli 16S rRNA gene by using a pairwise alignment and converted to conservative fragments.
Sequence similarity among the sequences were calculated by EMBOSS Align, a pairwise alignment algorithm (http://www.ebi.ac.uk/emboss/align).ac.uk/emboss/align
In the traditional 'progressive' alignment approach, this is done by calculating a pairwise alignment of profiles, but this procedure cannot be directly adapted to our segment-based approach.
IS elements, transposons, and their repeat elements were identified by doing a pairwise alignment using BlastN program against the ISfinder database (http://www-is.biotoul.fr/).fr/
Each read was compared to a reference set of sequences from the Los Alamos HCV database and was quality checked by comparing it in a pairwise alignment to the best reference match.
As the second strategy to identify phage-specific promoter elements, we developed an approach that is based on a pairwise alignment of the bacteriophage intergenic regions; this pairwise alignment was implemented by BLAST (balstn).
Rather than using all-by-all pairwise alignment (Niu et al., 2010) or clustering by alignment to dynamically created contiguous sequence fragments, we cluster individual sequence reads by grouping those reads mapped by Blat (Kent, 2002) alignment to an overlapping region in the reference genome of the host cell.
This is followed by automatic pairwise alignment of scans using a modified iterated-closest-points (ICP) algorithm and finally by a global relaxation procedure designed to minimize alignment errors across the entire statue (Pulli, 1999).
This is followed by automatic pairwise alignment of scans using a modified iterated-closest-points (ICP) algorithm and finally by a global relaxation procedure designed to minimize alignment errors across the entire statue.
This was followed by automatic pairwise alignment of scans using a modified iterated-closest-points (ICP) algorithm and finally by a global relaxation procedure designed to minimize alignment errors across the entire fragment [Pulli99].
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