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De novo annotation of the European eel transcriptome, both for the coding and for the non-coding fraction of transcripts, was obtained by a multistep procedure, starting with similarity search against main protein and nucleotide sequence databases, as detailed below.
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These goals have been addressed for TCCA-OAO in this study by adapting a multistep procedure that was recommended by Acquadro et al. [ 7].
For coupling of 89Zr to mAbs, we used a multistep procedure that has been developed by Verel et al. [33] using a succinylated derivative of desferrioxamine B (N-sucDf) as a bifunctional chelate.
The search algorithm of Glide is a multistep procedure based on a grid positioning followed by MC refinement; for Surflex, it is a Fragment/Surface based algorithm guided by a protomol, which is a negative fingerprint of the protein binding site; for FlexX, it is a fragment based algorithm; and for Gold, it is a genetic algorithm.
Implementation is a multistep procedure, which benefits from the adoption of international recommendations by local societies, as was the case for the 2010 EULAR recommendations.
The deuterated analogue of 2 (d-2) was synthesized via a multistep procedure and the intermediate deuterated complexes S1, S2, S3, S4 and S5 were isolated and characterized, see Supplementary Methods.
Automatic corrosion damage detection on metallic surfaces is a complex task which requires a multistep procedure.
Doing long sums in the absence of complementary actions or artefacts is a multistep procedure that quickly taxes working memory; congesting the phonological loop further handicaps performance.
Core flood process is a multistep procedure which included the initial brine injection, oil saturation, brine injection, and surfactant alternating gas injection (SAG).
The plasmid was constructed through a multistep procedure, essentially as described for the rhodopsin receptor [34].
Causal networks were inferred using a multistep procedure (Fig. 1).
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