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That is, the process is complicated, consisting of a two-step, two reaction-vessel followed by a multiple purification procedure including HPLC purification of the intermediate radiolabeled compound.
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Each positive PCR product was sequenced after DNA purification by a MiniElute PCR Purification Kit (Qiagen, Valencia, CA).
First, a regular PCR was performed followed by a purification step with the QIAquick PCR Purification Kit (250) (Germany, QIAGEN).
As a purification strategy, an electrocoagulation (EC) process followed by a biological purification step was applied.
The defects on the MWCNTs can be introduced by a special purification process [19 21].
followed by a LiCl purification step.
DNA was purified by two subsequent phenol chloroform:isoamylalcohol (25:24:1, pH 8) extractions and followed by a Qiaquick purification (PCR purification columns, Qiagen, Germany).
The homogeneity of YD1 was obtained by a two-step purification procedure, which resulted in 40-fold purification with 12% activity recovery.
This indicates that the affinity precipitation was a feasible method for the purification of HSA and electrophoretic purity of HSA can be obtained by a single-step purification.
Initial affinity column purification was followed by an additional purification using a GE Superdex 200 2.6/60 on an Akta Purifier (GE Healthcare) in PBS containing 0.1% sodium azide.
Each house has running water, supplied by a just-built purification plant.
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