Sentence examples for by a multiple cloning from inspiring English sources

The dual promoter expression vector was generated from the pCMV-tdTomato vector (Clonetech), in which the CMV promoter was replaced with a CAGG promoter followed by a multiple cloning site (MCS) and transcription terminator sequence.

We therefore constructed a NAN-GUS expression cassette in which the reporter genes were separated by a multiple cloning site, and in which the downstream GUS gene was out-of-frame with respect to the NAN gene, so that the vector could be used to characterize translational recoding events such as stop codon readthrough and, in addition, frameshifting.

A synthetic 2A peptide sequence followed by a multiple cloning site inserted using BsrG1/ NotI.

The L4440 (pPD129.36) vector contains two convergent T7 polymerase promoters in opposite orientation separated by a multiple cloning site [ 25].

The construct was cloned into pCM43 vector containing a 5xUAS- hsp70 promoter element followed by a multiple cloning site, a SV40 element and mini- white gene.

In the related RIVFRT.MCS.pA.FRT MCS3 vector, QF was replaced by a multiple cloning site distinct from MSC (MCS3) to allow insertion of another cDNA downstream of the one flanked by FRTs.

The heat-inducible hairy gene was created by introducing a multiple cloning site containing Kozak sequence, initiator ATG and HindIII/BglII sites into the 5′ portion of the hairy ORF in the pCaSpeR-hsh using EcoRI/BstEII sites as described previously (Li and Arnosti, 2011).

In brief, the AAV constructs (pAAVsp and AAV; Salk Institute) contained a cytomegalovirus promoter, a synthetic intron flanked by splice donor/splice acceptor sites, and a multiple cloning site terminated by a β-globin polyA sequence.

We completed our expression vector by cloning a selectable marker, a multiple cloning site, a promoter and reporter genes into the plasmid (Fig. 1).

The exon trap vector pET01 (MoBiTec) contains two exons separated by an intron sequence containing a multiple cloning site (Fig. 4).

The wild-type and mutant bacterioopsin genes were cloned into pGEMHE, providing a multiple cloning site surrounded by the noncoding regions of β-globin from Xenopus laevis.