Sentence examples for by a multiple alignment from inspiring English sources

A primer and probe set for qPCR was designed by a multiple alignment of conserved amino acid sequences encoding the large subunit of aniline dioxygenase.

Here we report the isolation of a partial coding sequence of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene ortholog in Haloxylon salicornicum, a desert plant native to Kuwait, by designing a non-degenerate consensus primer pair based on conserved regions generated by a multiple alignment of orthologous GAPDH DNA sequences derived from closely-related species.

Since each orthologous group is represented by a multiple alignment of protein sequences, we need a very fast algorithm to compare multiple alignments.

Identification of the labeled compartments was performed on composite images obtained by a multiple alignment ('stitching') of 25 36 high-resolution images per nucleus.

When these proteins were analyzed by a multiple alignment, the conservation in sequence between the protozoa eIF3b orthologues and those from multicellular eukaryotes (fungi, mammals and plants) is more pronounced at the C-terminal WD repeat domain and within the protein's center, whilst the conservation within the RRM domain is more restricted to its N-terminal end.

Aligning step: The 1046 groups were aligned by Muscle, a multiple alignment program [ 23].

On the basis of maximum identity score, the sequences were selected and aligned by using a multiple alignment software program such as Clustal W. Distance matrix was generated using RDP database and the phylogenetic tree was constructed using MEGA 5 (Tamura et al. 2013).

This profile was constructed by using a multiple alignment of amino acid sequences corresponding to the known α-helical coiled coil domains found in the Protein Data Bank (PDB) [24], release 2006.

Our software program can use these sites as anchor points by creating a multiple alignment respecting these constraints.

The BmrD/TM288 alignment was instead obtained by manually optimizing a multiple alignment involving sequences of BmrC, BmrD, TM287, TM288, and SAV1866 (Dawson and Locher, 2006; Figure 1 figure supplement 1C), which was generated using Clustal-Ω (Sievers et al., 2011).

The evolutionary rate of AM proteins was assessed by carrying out a multiple alignment (MA) of each human protein with its orthologs from a series of model organisms (Mus musculus, Gallus gallus, Xenopus laevis, Danio rerio, Takifugu rubripes, Drosophila melanogaster, Caenorhabditis elegans, Dictyostelium discoideum, Bacillus subtilis, Methanococcus jannaschii).