The absorbance was measured by a SynergyMx Multimode Reader at 520 nm.
The fluorescence intensity was monitored in real time by using a BMG Labtech PolarStar Omega multimode reader fitted with 620-10 and 665-10 excitandon and emission filters, respectively, at an excitation wavelength of 622 nm and an emission wavelength of 670 nm.
Then, cells were incubated with 10 μM DCF-DA for 15 min at 37°C, and the DCF fluorescence was measured by using a multimode plate reader (Ex 485 nm and Em 535 nm).
Luciferase activity, as a marker of cell proliferation, was measured by Analyst GT Multimode Reader (Molecular Devices, Sunnyvale, CA) and plotted against control siRNA.
Luciferase activity, as a marker of cell viability, was measured by Analyst GT Multimode Reader (Molecular Devices, Sunnyvale, CA) and plotted against control siRNA.
Luciferase activity, as a marker of cell viability, was measured by Analyst GT Multimode Reader.
As described previously [ 20], BM-MSCs (n = 5) were lysed, and the amount of DNA was measured with Quant-iT PicoGreen dsDNA assay kit (Invitrogen) using a SynergyMx Multimode Reader (BioTek, Winooski, VT, USA) as described by the manufacturer.
Finally, the mixture was examined by fluorescence photometry (excitation at 400 nm, emission at 505 nm) in a Modulus Multimode Reader.
The density was determined using a microplate multimode reader (Turner Biosystems, Sunnyvale, CA, USA) set at 450 nm.
The relative light units of luciferase gene expression were determined by Modulus Microplate Multimode Reader.
