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Lipids were extracted by a modified protocol of Bligh and Dyer (1959) from freeze-dried cells.
Whole-mount mRNA in situ analysis was performed by a modified protocol [19] using a digoxigenin-labeled antisense probes.
For DC immunization in SEA/Pa experiments, DC were generated by a modified protocol.
This was synthesized by a modified protocol based on previously reported work.
The purification of the lysosomal fractions for CMA assays was performed by a modified protocol based on methods previously described (Kaushik & Cuervo, 2008; Malkus & Ischiropoulos, 2012).
Substrate polyacrylamide gel electrophoresis was carried out by a modified protocol of Heussen and Dowdle, using the gelatine Ready Gel Zymogram 10% (Bio-Rad Laboratories).
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In contrast, the detection of cell membrane proteins embedded in the dense extracellular matrix was successfully accomplished by using a modified protocol by Ozbey and colleagues [ 21] (see Materials and methods).
In the present study, ischemia was created in the vascular bed of chick embryo by using a modified protocol as mentioned by Vos et al., 2003 where they studied the hemodynamics due to blockade in right vitelline vein [51].
High-level transfection efficiency for eIF3f knockdown by shRNA in primary satellite cells was achieved by using a modified protocol for Lipofectamin 2000.
The prosthetic group [18 F]11 was synthesized by following a modified protocol of Li et al. [22] using tetrabutylammonium hydroxide as base during 18 F-fluorination instead of the Kryptofix 2.2.2/potassium carbonate system.
CoA esters were analyzed as described [31] or by applying a modified protocol with significantly improved desalting of the samples.
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