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Targeting of the dRecQ4 locus was achieved by a modified procedure described by Rong and Golic [47] and Egli and colleagues [48], [49].
The dipeptide L-Ala-D-Glu was synthesized chemically according to Sachs and Brand [46] and the tripeptide L-Ala-γ-D-Glu-L-Lys was synthesized by a modified procedure of Schmidt et al. [47].
Covellite CuS nanoplates were synthesized by a modified procedure previously reported by us.
Macrophages and neutrophils were analyzed by a modified procedure for muscle-associated cells, as described previously (27).
The concentrations of hydroxyl radicals were measured by a modified procedure based on the hydroxylation of sodium salicylates by hydroxyl radicals, leading to the production of 2,3-dihydroxybenzoic acid and 2,5-dihydroxybenzoic acid [ 12].
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To evaluate the inoculum effect of hVISA strains detected by the Macro Etest Method, a modified procedure involving Brain Heart Infusion agar contain vancomycin 4 mg/L BHIAV4 method was performed as described previously [ 6].
The isolation of DNA was carried out by a slightly modified procedure reported by Choudhary et al. (Choudhary et al. 2003).
[Co en 3]Cl3∙ 3H2O was prepared by a slightly modified procedure described in the literature [81].
The coupling cycle was carried out by a slightly modified procedure consisting in the following steps: (1) washing with 2.5 mL of (ACN/DMF) (1 : 1, v/v); (2) coupling 20 minutes; (3) washing with 2.5 mL of ACN/DMF (1 : 1, v/v); (4) capping with 5% of acetic anhydride and 6% of 2,6-lutidine in DMF, 2.0 mL; (5) washing with 2.5 mL of ACN/DMF (1 : 1, v/v) and 2.5 mL of DMF.
Simons' stain was performed according to the modified procedure by Chandra et al. [ 65].
Total genomic DNA was extracted from individual mosquitoes following a slightly modified procedure described by Collins [26].
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