Sentence examples for by a modified alkaline from inspiring English sources

The constructs were amplified in large-scale bacterial cultures from which the fragments were obtained by a modified alkaline lysis procedure and subsequent digestion with EcoRV.

Plasmids were isolated and purified automatically by a modified alkaline lysis procedure.

BAC DNA was extracted by a modified alkaline lysis method [ 59] with the following changes: BAC clones were cultured for 18 hr in 3 ml of LB media containing 12.5 μg/ml of chloramphenicol and harvested by centrifugation followed by decanting of the media.

Objective: To correlate sperm variables with sperm DNA fragmentation, as assessed by using a modified alkaline comet assay for sperm smears.

Consequently, DNA was extracted from frozen whole blood by using a modified alkaline lysis method adapted from an avian cell culture DNA extraction method (10 ).

Plasmids were isolated by using a modified alkaline lysis protocol [ 41], and inserts were sequenced using vector specific primers (M13F and T7) on an ABI Prism 3700 automated DNA sequencer (Applied Biosystems, Foster City, CA).

BAC DNA was obtained using a modified alkaline method followed by purification using the Microplate Unifilter+Uniplate Devices from Whatman Inc.

BAC DNA was isolated using a modified alkaline lysis method followed by purification.

BAC DNA was isolated using a modified alkaline lysis method followed by purification with 70% ethanol [ 50].

Preparation of plasmid DNA was performed utilizing a modified alkaline lysis protocol followed by purification through anion exchange column (Qiagen, Valencia, CA).

The aim of this study was to test the combined effect of a quality-controlled red clover extract (RCE) standardized to contain 40% isoflavones by weight (genistein, daidzein, biochanin A, and formononetin present as hydrolyzed aglycones) together with a modified alkaline supplementation on bone metabolic and biomechanical parameters in an experimental model of surgically-induced menopause.