Sentence examples for by a mobile phase from inspiring English sources

Samples were eluted isocratically from a Synergi 4-μm MAX-RP 100 Å column (2.0 × 50 mm; Phenomenex) by a mobile phase consisting of 10 mM ammonium formate in water and methanol (30 70 [vol/vol]) at a flow rate of 0.20 mL/min.

The chromatographic conditions were; reversed phase column (Nucleosil C8, 150 × 4.6 mm, 5 μm), isocratic elution by a mobile phase consisted of 20% acetonitrile containing 0.1% trifluoroacetic acid, and the flow rate was set at 1 mL/min.

1.7 μm).The best peak resolution along with high sensitivity was achieved with an isocratic elution by a mobile phase comprising of acetonitrile: 10 mM ammonium acetate (60 40) at a flow-rate of 0.3 mL/min.

The best resolution of peaks were achieved with an isocratic elution by a mobile phase comprising acetonitrile:water:formic acid (65 35:0.1%, v/v/v) at a flow-rate of 0.2 mL/min, on Acquity UPLC BEH™ C18 column (50 × 2.1 mm, i.d. 1.7 μm).

Chromatographic separation was achieved on a Zorbax Eclipse XDB-C8 column (150 mm × 4.6 mm i.d., particle size 5 μm, maintained at ambient temperature) by a mobile phase consisted of acetonitrile and 50 mM potassium dihydrogen phosphate buffer (10 90, v/v) with apparent pH of 3.5 ± 0.1 and a flow rate of 1.0 ml/min.

Chromatographic conditions are: reversed phase column (Nucleosil C8, 150 × 4.6 mm, 5 μm), isocratic elution by a mobile phase consisted of 20% acetonitrile containing 0.1% trifluoroacetic acid, the flow rate was set at 1 mL/min, the detection was UV at 254 nm. Figure 3 1 H-NMR (A) and 13 C (B) spectra of glutaryl-LND derivative.

dRI baseline fluctuations caused by temperature programming were minimized by using a mobile phase heater to thermostat connecting tubing.

The chromatographic separation was achieved on a Symmetry shield C18 column (250 mm × 4.6 mm, 5 μm) by employing a mobile phase consisting of methanol potassium phosphate buffer (0.05 M) mixture (15:85, v/v) (pH 3.5) at a flow rate of 1 ml min−1; detection at 255 nm.

Separation and resolution of picrosides was carried out on a reversed phase (C-18) column by using a mobile phase of methanol and water (40 60 v/v).

The flow rate was 0.6 mL min−1 and the temperature was kept at 22°C. 100 µL of sample solution (~2 mg mL−1 protein of 95% or higher purity) was separated by applying a mobile phase containing 10 mM Tris HCl (pH 7.2), and 500 mM sodium chloride.

Separation was achieved by using a mobile phase of methanol/water (90 10 vol/vol) and at flow rate of 1 mL/min.