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The Andean population was likely founded by a mixed sample of Iberian breeds; however, by combining the descendants of those breeds in the comparison group, many of which have undergone strong subsequent artificial selection since their introduction to the Americas, one effectively makes a comparison to a more heterogeneous out-group.
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The proportion of waxy DNA in a mixed sample could be determined by calculating the cycle differences in the qPCR assays using waxy- and non-waxy-specific primers without further calculation.
This finding [ 5] is confounded by a small, mixed sample of healthy controls and LBP patients, and absence of a direct stretch tolerance measure.
Batch experiment was carried out by adding 50 cm3 of mixed sample in a conical flask taking 1.0 g of CA/TPC under optimized conditions as described.
Size-based growth rates of immature green turtles inhabiting a foraging ground at Akumal Bay (20°24′0″N and 87°19′16″W) were obtained by using a mixed longitudinal sampling design from historic mark recapture data (2004 2014).
Sample selection was done by a mixed method of randomised cluster sampling and snowball sampling approaches because of unavailability of the entire list of Turkish inhabitants in London.
When introduced early in the sample preparation, label-based methods are preferred for analyses that require extensive prefractionation/enrichment approaches, as lack of reproducibility of the above methods can be compensated for by a unique processing of the mixed sample.
Technical replication was completed by independently processing the mixed sample of 20 individuals starting from the low molecular weight RNA isolation step.
For each variety, three biological samples, each represented by the mixed samples at the same phonological growth stage from at least three individual spikes, were used for qRT-PCR.
After the NGS assay followed by de novo assembly, 8 contigs were generated for the H5N1 and H3N2 viruses, and 15 contigs were generated for the mixed sample, supported by a high coverage rate of reads (Table 2).
A total of 1688 respondents were recruited by multi-stage mixed sampling in 1000 households in 18 communities of Hangzhou.
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