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The Closing operation is composed of two steps: a maximum filtering followed by a minimum filtering (fig. 6).
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An Opening is the opposite operation, a minimum filtering followed by a maximum filtering.
Filtering by a minimum expression level obscures the influence of extreme variability of low expressed genes.
Post-translationally modified peptides were filtered by a minimum Mascot Ions Score of 15 and validated by manual inspection.
Data were filtered by a minimum mean of 20 reads mapped per gene and only those genes with a fold difference between groups higher than 1.2-fold or lower than −1.2-fold were retained.
These statistics result from filtering reads by a minimum mapping quality of 20 with samtools, as suggested to remove ambiguous mapping (http://samtools.sourceforge.net/).net/
The derived performance indices are then calculated using the gains determined by a minimum-variance (MV) filter criterion [31], which is the optimal gain design for the general α- β- γ filter.
From all predictions based on RefSeq transcript IDs, we filtered out only miRNA-transcript relations that were predicted by a minimum of four different tools.
The SPPs were filtered to allow zero missing and zero inconsistent data, coverage by a minimum of two probes at the interrogated position, a minimum SPP width of 4 bp and a SFPdev ratio of 1.2.
Further filtering into high confidence SNPs was performed by screening for at least two alleles represented by a minimum depth of three reads per nucleotide call per allele.
Conversely, upward communications have to be pumped and piped, with a minimum of filters, in order to be effective.
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