By the definition of Γ j we know that v is a minimal vector in V j, so v ≼ w, which contradicts w ≺ v.
Formally speaking, a vector is called a minimal vector in if there exists no vector in such that ; it is called a weak minimal vector if there exists no vector in such that.
On the other hand, suppose that ( X, M ) is a minimal vector space which is not a topologicalvector space.
The latter vector is referred to as a minimal vector since it retains only the viral components required for replication, including the RNA-dependent RNA polymerase.
By using the result of Antczak [13] and the definition of a weak minimal vector, we obtain the following proposition.
Therefore, is a weak minimal vector in the set W. The converse part is proved similarly.
Resolution of the cointegrates was achieved by conjugative transfer into a recipient strain overexpressing the tnpR resolvase gene, resulting in the release of a copy of the cDNA-carrying minimal vector with an insertion of the mini-Tn 1000:: nptII.
Plasmid pACT contains a luciferase cassette driven by a minimal, enhancerless promoter, into which vector proviruses are inserted upstream for evaluation by luciferase assays and northern blots.
In cell suspensions, a minimal PVX vector retaining only the viral RNA polymerase gene yielded 6.6-fold more GUS than an analogous full-length PVX vector.
Before transformation, the plasmid DNA was linearised by digestion with a rare-cutting restriction enzyme, SdaI, that cuts in the minimal vector just outside of the cDNA inserts (Fig. 2).
