Gonzalez, I., Mateos-Langerak, J., Thomas, A., Cheutin, T. & Cavalli, G. Identification of regulators of the three-dimensional polycomb organization by a microscopy-based genome-wide RNAi screen.
The role of these proteins was tested by a microscopy-based RNAi screen in S2 cells, and our approach consisted of 3 phases (see Figure 1C): The first was a primary screen designed to detect changes in the pattern of the inducible GFP-tagged Golgi resident enzyme Fringe.
The filtered light reflecting SSD is collected by a confocal microscopy system.
Ca2+ levels were determined by a fluorescence microscopy.
The staining of the cells was observed by a fluorescence microscopy.
After three washes in PBS, cells were visualized by a confocal microscopy as described previously [22].
All these dynamical processes cannot be easily followed by a single microscopy technique or by single molecule approaches.
The fluorescent staining was recorded by a confocal microscopy (Olympus).
The cells were mounted on the slide and observed by a fluorescence microscopy (Carl Zeiss).
Protein localization was visualized by a confocal microscopy (Olympus Fluoview, Tokyo, Japan).
