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Afterward the beam is reflected by a mirror and focused by a microscope objective (NA = 0.3).
Passing through the microlens, the transmitted speckle pattern is collected by a microscope objective and measured by a CCD.
The excitation is then tightly focused by a microscope objective (60 ×, NA 1.27, water), which also collects the back-scattered light.
Using spatial light modulators we can engineer the wavefront of a laser beam in such a way that, once focused by a microscope objective, it produces an almost arbitrary light intensity distribution.
The beam was then focused by a microscope objective lens (10 ×, NA = 0.25, Leica) into a cuvette containing FNDs or FND-5GNP conjugates in deionized water.
The first diffraction order is directed towards the animal, and the backscattered light is collected by a microscope objective (10 X) and imaged onto the camera.
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Although the MPM stack is obtained by scanning a microscope objective in the Z direction, the thickness of the sample obtained from the MPM cross-sectional image is not exactly the physical thickness due to the multilayer refractions in the sample arm.
The imaging system consisted of a 20X wide field microscope eyepiece (Model NT39-696, Edmundseparated separated by 160 mm from a microscope objective (60X 0.85NA DIN Achromat objective, 160 mm tube length, Model NT38-340, Edmunds Optics).
Images of the smears were recorded by a 20× microscope objective and by the iPhone 2G microscope equipped with a 1 mm ball lens.
PL line scans were performed on a suitably dried structure by illuminating from the transparent substrate side using a 410 nm diode laser focused by a 40X microscope objective, and collecting the PL in reflection mode.
Further magnification along with corresponding reduction of the field of view to ~3 mm was achieved when the camera objective was replaced by a 4x microscope objective.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com