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The laser beam was focused by a microscope equipped with a × 50 objective.
The laser beam was focused by a microscope equipped with a × 50 or × 100 objective, leading to a spot diameter of 2 or 1 μm.
Visual control was achieved by a microscope equipped with an infrared DIC system (Leica DM6000, Leica, Mannheim, Germany) and an IR objective (HCX APO L 20x/1.0 W; Leica).
The dimension of each section and the thickness of the bonded layer were determined by a microscope equipped with a digital micrometer.
The cell images were observed by a microscope equipped with fluorescence light source (FLoid Cell Fluorescence Imaging Station, Invitrogen), and the cell micrographs were taken with a CCD camera.
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This combination of two kinds of twinning, although best seen by means of a microscope equipped to use doubly polarized light, is sometimes discernible macroscopically.
The terpolymers were characterized using 1H NMR spectroscopy and SEC measurements; the thermal properties were studied by TGA and DSC investigations as well as the self-healing properties by real-time analyses using a microscope equipped with a camera.
Images were captured by a DM2500 microscope equipped with a DFC350FX digital camera (Leica Microsystems, Wetzlar, Germany).
Digital images were acquired by a fluorescence microscope equipped with a DC200 digital camera (Leica Microsystems, Wetzlar, Germany) and were analyzed by Leica Qwin image analysis software (Leica Microsystems).
Immunofluorescence was acquired using a scanning sequential mode to avoid bleed-through effects by an IX81 microscope equipped with a confocal scan unit FV500 with three laser lines: Ar-Kr (488 nm), He-Ne red (646 nm), and He-Ne green (532 nm, Olympus, Tokyo, Japan) and a UV diode.
The surfaces were analysed by using a JEOL-6500F microscopequippeded with a field emission gun (FEG) coupled with an energy dispersive X-ray (EDX) spectrometer.
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