Then, the absorbance was measured by a microplate reader at 490 nm.
The sensing mechanism was first validated by a microplate reader method and then applied to the microfluidic platform.
After incubating at 37 °C for 4 h, the absorbance was measured at 405 nm by a microplate reader (Spectrophotometer 1510, Thermo Fisher Scientific, Vantaa, Finland).
The absorbance of each well (OD value) was measured at 570 nm by a Microplate Reader (Bio-Rad, Hercules, CA, USA).
Finally, the solutions were retrieved and the absorbance was measured by a microplate reader (Biotek, USA) at the wavelength of 570 nm.
The supernatant was used to evaluate the fluorescence intensity by a microplate reader (GENios, Tecan, Switzerland).
The luminescence in each well was measured by a microplate reader 16 h after the transformation.
After 30-min incubation, absorbance at 570 nm was read by a microplate reader.
The intensity was measured by a microplate reader (BioRad, USA) at a wavelength of 450 nm.
The absorbance was then measured at 570 nm by a microplate reader.
The absorbance at 492 nm for each well was recorded by a microplate reader.
