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The gradient was generated by a microinjector connected to a glass capillary needle filled with 1 µM cAMP (Femtotips, Eppendorf, Germany).
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Locomotion was observed by a webcam connected to a screen.
ZIP or saline were infused into the dorsal hippocampus (1 μl per hemisphere) via a microinjector (28 gauge) connected to a microinfusion pump (Stoelting Co., Wood Dale, IL, USA) at a rate of 0.25 μl per min. The injector remained connected for an additional 1 min to allow for drug diffusion away from the tip of the cannula.
The infusion was performed with a microinjector (28 gauge) connected to a Hamilton syringe with plastic tubing at a rate of 0.25 μl/min.
For evaluation and demonstration purposes, the microinjector was connected to a 75 μm ID capillary and UV absorbance detector.
Zebrafish embryos at one-cell stage were mock-injected or injected with 9.2 nl of pIR8 at the concentration of 150 ng/μl by using a microinjector (Leica EZ4 nanoliter 2000).
New cultures [37] and early chick (EC) cultures [38] were prepared, and transfection was achieved by electroporation of 0.5 μl plasmid DNA at a concentration of 1.0 mg/ml, microinjected into the space between the vitellin membrane and the epiblast, next to the anterior primitive streak, in HH2-3 embyyos, by using a microinjector (FemtoJet, Eppendorf).
A glass pipette (tip diameter ∼30 µm) connected to a microinjector (IM-9B; NARISHIGE) was used for the tracer injection.
The solutions to be injected were loaded into a glass microelectrode connected to a microinjector (picospritzer II, Parker Hannifin Instrumentation).
Using a glass capillary (tip diameter 80 100 µm) connected to a microinjector (Kopf Instruments, Tujunga, CA), rats were injected with 0.5 µl (10, 000 cells per injection) of the iPS (n = 3) or proliferating H9 cells in DMEF/F12 media.
The distinct and well separated colonies of 1 3 cells that formed were then individually aspirated with a pulled glass capillary connected to a microinjector (Nanoject 2000, WPI, Germany), transferred to a single well of 96 well plates in 200 µl of tissue culture medium.
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