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Missing SNP genotypes were imputed by a method adapted from [ 51].
DHFR activity was measured by a method adapted from published procedures [22,23].
Hybridization between probe and target DNA was detected by a method adapted from the protein ELISA technique.
The cellulase system from T. reesei, ATCC 26921 (purchased from Sigma-Aldrich), was immobilized onto Si chips, by a method adapted from Tebeka et al. [ 25].
The loss of expression of intimin was confirmed using SDS-PAGE followed by silver staining and Western blotting by a method adapted for semi-dry transfer [ 34].
Total RNA was isolated from each set of frozen internodes (5 g) by a method adapted from Ragueh et al. [ 117]and developed in Guillaumie et al. [ 43].
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PFGE was performed on 573 viable isolates of serogroup A, B, C, W135, and Y meningococci by using a method adapted from Popovic et al. (25 ).
To precisely localize cenH3-containing nucleosomes and identify potential kinetochore attachment sites, we digested chromatin from mixed-stage embryos with micrococcal nuclease (MNase) and solubilized the majority of the chromatin by cavitation, a method adapted from Jin and Felsenfeld, 2007.
Liver malondialdehyde (MDA) was determined by HPLC using a method adapted from Khoschsorur et al. [ 14].
Bovine fibroblast cells were isolated from cow aortas by collagenase treatment using a method adapted described earlier [ 27].
Intracellular levels of pteridines were quantified by HPLC analysis using a method adapted from Fukushima and Nixon [20] as described previously [21].
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