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This tissue specific expression of the isoform is often accompanied by a lowered expression of the other isoform(s).
The RUNX1/Runx1 knock-out/silencing can be traced by a lowered expression in human knock-out cells (indicated by green boxes near the Ensembl ID ENSG00000159216) as well as in mouse (background of the box for Ensembl ID ENSMUSP00000109589).
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Previous experiments in mice liver lobules suggest that the perivenous genetic program is switched off, and the periportal program switched on, by a WNT inhibitor or by lowered expression of β-catenin [ 26].
Conversely, inhibition of TRIM16 protein expression by a specific siRNA lowered expression levels of procaspase-2.
This is reflected at the undifferentiated state in OSKMV cells by notably lowered expression of OCT3/4 (POU5F1), TERT and SALL4.
This result was not caused by lowered expression levels of RasGAP or Aurora B in RG27-expressing cells.
In contrast, the peritoneal B2 cells, characterized by a lower expression of IgM, higher expression of IgD and an absence of CD5, express CD38 at similar levels to B cells in the other B cell compartments analyzed.
This observation may be caused by lowered expression or reduced function of these microRNAs in androgen-independent prostate samples.
Altogether, our data indicate that SSQ1 was subjected to divergent evolution driven by lowered expression levels and functional diversification, but that critical function was maintained by purifying selection.
However, we did verify a correlation for the FMV cry2Ab2 transgene, where both FMV promoter and cry2Ab2 sequences showed higher levels of CG and CHG methylation in the stacked event accompanied by a lower expression of the cry2Ab2 transgene.
The lack of increased grain number by inhibiting CKX2 expression, as observed in this study, may be caused by a lower expression of shRNAs in the inflorescence (i.e. a lower amount of cytokinins accumulated) to trigger differentiation of more florets in the panicles.
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