Therefore, the photoreceptor cell appears to tolerate some degree of rhodopsin mislocalization by low level expression of these trafficking mutants.
A comparison of EDNRB in four cutaneous melanoma cell lines originating from metastases, with three cell lines from primary tumours has shown the metastatic tumours to be characterised by low level expression of EDNRB (Kikuchi et al, 1996).
In Drosophila loss-of-function mutations in the LKB1 gene caused defects in polarity of the oocyte, and this was rescued by low level expression in the germ line of wild-type LKB1, but not by mutation of the residue homologous with Ser (Ser) [ 29].
The duration of protein expression from genes delivered via plasmid DNA delivery is normally transient, lasting for only a few days, followed by a period of prolonged yet low level expression.
This cannot be explained by a low level of expression of TNF-α since Cq values obtained by qPCR with TNF-α-2 primers were around 24 cycles.
Since HHFs express detectable levels of endogenous MARCH-VIII and MARCH-IV (Fig. 7A), we wondered whether the steady state level of CD44 or CD81 was affected by the constitutive, albeit low level expression of these ubiquitin ligases.
See inset in Additional File 2 for relative numbers of genes exhibiting high, medium, and low level expression by each method.
This could be explained either by very early and short IFN expression (before day 3) [ 35] or by a low level of IFN expression produced by plasmacytoid DCs, which were not inhibited by CSFV [ 2, 57, 67] and probably undetectable by microarray due to the relatively low concentration of these cells in whole tissue samples.
Another domain characterized by a low level of Cux2 expression emerged in Calbindin+ neurons of the developing DG blades.
For UCAL-P19 (named uterocalin in horse [ 37]) a low signal was found all along the epididymis by PCR, suggesting a low level of expression for this gene and the differential expression could not be confirmed.
