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The most relevant differences from our structure are represented by the long arm that in HpPgdA protrudes from the monomer core, by a loop region (205 217) connecting helices α-7 to α-8, which is definitely longer in HpPgdA, and by the loop connecting helices α-1 to α-2, which in HpPgdA includes only five residues, whilst in PuuE is about 27 residues long.
The HLH region contains two amphipathic α helices separated by a loop region of variable length.
A P-domain is basically the 2TM domain and composed of two transmembrane helices connected by a loop region referred to as the P-loop.
A detailed domain analysis according to [ 28] revealed a basic DNA binding region (aa 397 to 406) N-terminal to two alpha-helices separated by a loop region (aa 409 to 461).
The basic domains contact the major groove of the DNA, and the HLH domains, comprising helices 1 and 2 (H2, H2) joined by a loop region, form a parallel left-handed four-helix bundle.
To systematically identify all inc genes in fully sequenced Chlamydiae genomes we designed a biocomputational approach based on the presence, in all Inc proteins, of at least two transmembrane domains separated by a loop region ([ 16- 18] and this study).
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The ligand-free structures of both MALT1-P and TbMCA2 revealed that the active sites were blocked sterically, and so most probably autoinhibited, by a residue on a loop region in the structures (Gln on L5 in MALT1-P and Tyr on the N-terminal region of TbMCA2).
Our modeling revealed that the enzymatic core is formed by a twisted, six-stranded β-sheet flanked by six helices (α1-α6) linked through a loop region to the cell wall binding domain which consists of two anti-parallel β-sheets.
This position is close to a loop region formed by residues Y390 to N394, which is absent in all of the 30 closest homologs of GOOX (analysis performed at http://consurf.tau.ac.il/).
It is also worth noting that LepB Arg444 is situated in a loop region rather than supplied by a helix as observed with those in Gyp1p and VirA/EspG, highlighting an additional structural difference between LepB and known RabGAPs.
The bHLH transcription factors are named after their highly conserved domain (about 60 amino acids long) that consists of a DNA-binding basic region (b) followed by two α-helices separated by a variable loop region (HLH) [ 1].
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