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The TPR and GPR domains are separated by a linker sequence in the middle.
It is a typical two-domain response regulator of the NarL subfamily [5] and its N-terminal domain that contains a phosphorylation site, Asp54, is connected to the C-terminal DNA binding domain (DevRC) by a linker sequence [16] [19].
The final constructs code for the α1α2 and the β1β2 domains, respectively, followed by a linker sequence (SSADL), a thrombin site (LVPRGS), a leucine zipper (acidic for the α chain, basic for the β chain) and a hexa-histidine tag (C-terminal His-tag).
Padlock probes consist of end segments, complementary to a target sequence, connected by a linker sequence.
The GST and EGFP coding regions were connected by a linker sequence (5′-CAT GGA TAT CGC A-3′).
Interface 2 is connected to interface 3 by a linker sequence that has a PXXΦP motif (Φ: any hydrophobic residue).
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(H – K ) 10-nm thick electron cryotomographic slices of E. coli cells expressing engineered protein constructs based on FtsZ(D212A) (see also Figure 1 figure supplements 3,5 and Supplementary file 1, Table B ). (H ) Extending the C-terminal linker of FtsZ by inserting a linker sequence pushes the filaments further away from the IM (distance changed from 16 nm to a somewhat variable 16 21 nm).
They contain two arms separated by a poly(A) linker sequence and followed by a poly(A) tail.
The R115P mutation identified by exome sequencing lies in a linker sequence between the adaptor-binding and the Ras-binding domains (24).
The scrambled peptide for Hsp70 (TPTYRASCFYHYK) was also hybridized by the R11 peptide with a linker sequence as follows: RRRRRRRRRRRGGGTPTYRASCFYHYK R11-Hsp70scramblee).
To further improve the cytotoxic activity of Antp-TPR toward cancer cells, we investigated the effect of a Hsp70-targeted peptide, which was made cell-permeable by adding the polyarginine with a linker sequence, on the cytotoxic activity of Antp-TPR in breast cancer cell lines.
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