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The progress curve is well described by a single exponential phase followed by a linear steady state.
A burst of NADH production is also observed when the CpIMPDH reaction is monitored by fluorescence, and again the progress curve is well described by a single exponential burst phase followed by a linear steady state.
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When the reaction was monitored by fluorescence, the progress curve exhibits three phases, a lag followed by a burst and a linear steady state, which are well described by eq 7. Replots of the values of k obs1 and k obs2 versus [NAD+] are both hyperbolic, with maximum values of 23 ± 1 s−1 and 12 ± 1 s−1 for the lag and burst phases, respectively (Table 4).
When the reaction was monitored by absorbance, the progress curves display a burst of NADH production and are well described by a single exponential with a linear steady state.
Overall, our experimental profiles match those expected from the model and can be almost completely described by a single exponential approach to a linear steady state (Supplementary Figure S5).
Data from that source indicates that the fraction of profits on corporate income taken by federal, state, local and foreign taxes peaked during World War II and has shown a fairly linear, steady and steep downtrend ever since.
The individual rate constants are shown in Scheme 1. Signal response factors for the fluorescence data were calculated for each concentration of NAD+ to account for inner filter effects by normalizing the linear steady-state regions with the absorbance data.
The modelling procedures are all twofold; consisting of a solution to a steady-state heat conduction problem followed by a linear static solution.
Res., 35 853–870.], (Fig. 2) can be generated either by a linear diffusion equation under transient conditions or a nonlinear diffusion equation under steady state conditions.
While in animals the Patlak approach may not be valid, in humans a steady state appears to be reached which is accompanied by a linear portion of the Patlak plot justifying its use for quantification purposes [ 78].
The model is expressed by a linear differential equation (1) where IGn (mmol/l) is normalized interstitial glucose equalling blood glucose at steady state; the time constant τ (min) represents the delay between blood glucose and normalized interstitial glucose.
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