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Three µg nuclear extract was used for SRE transcription assay by a kit from Marligen Bioscience (catalog # 11944-096 and 11906-100) according to manufacturer's instructions.
The serum levels of alanine transaminase (ALT), aspartate transaminase (AST) and total cholesterol (TC) were determined by a kit from ShenSuoYouFu (Shanghai, China).
IL-18 was quantified by a kit from Medical & Biological Laboratories Co. , Ltd
Serum ALP activity was determined by a kit from BioSystems, SA.
The protein was analysed by a kit from Bio-Rad (Sundbyberg, Sweden) using bovine albumin as a standard.
Plasma glucagon was measured with a kit from Euro-Diagnostica (Arnhem, The Netherlands), plasma cortisol with the Count-A-Count Cortisol kit (DPC, Los Angeles, USA) and fructosamine by a kit from Spinreact (Sant Esteve De Bas, Spain).
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Cyclic GMP was measured by using a kit from Cayman Chemical Ann Arborr, MI).
UN level in all the experimental samples were measured by using a kit from Span Diagnostics Limited, India.
T cells producing IFN- γ were enumerated by ELISPOT using a kit from BD-Biosciences (San Diego, CA, USA) according to manufacturer instructions, by culturing 8 × 10 splenocytes from immunized mice in the absence/presence of peptide p1073 (10 μg/mL), NS3 protein (0.1 μg/mL), or culture medium (negative control).
Systemic IL-10 levels were measured by ELISA using a kit from BD Biosciences (Cat 555157) following manufacturer instructions.
The protein levels of MCP-1 in conditioned media were measured by ELISA using a kit from R&D Systems (Minneapolis, MN).
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