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The AlkA concentration dependence was fit by a hyperbolic dependence, with a maximal single-turnover rate constant (kmax) of 0.27 ± 0.01 min–1 (0.0045 s–1) and a K1/2 value of 29 ± 3 nM.
The concentration dependence of the single-turnover rate constant was fit by a hyperbolic dependence (eq 4) 4where kmax is the maximal single-turnover rate constant, E is AlkA, and the K1/2 is the concentration of AlkA at which kobs is 50% of the kmax value.
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The value of k obs for the burst phase displays a hyperbolic dependence on NAD+ concentration.
In agreement with previous findings [ 19], the β-carotene bleaching rate showed a hyperbolic dependence on β-carotene concentration.
Previous stopped-flow fluorescence assays investigating matched dNTP incorporation showed that both the fast and slow fluorescence transitions demonstrated a hyperbolic dependence on dNTP concentration (24, 30).
Hence, the simplifying assumption of a hyperbolic dependence of the PFK activity on ATP as a substrate seems to be reasonable.
The first phase (kobs1) shows a rapid initial decrease in the absorbance of the oxidized flavin and a hyperbolic dependence on NADH concentration.
(E ) The observed rate constants for ATP binding to heat-treated β-cardiac display a hyperbolic dependence upon the incubation with EMD 57033.
Assuming rapid substrate binding, the observed rate constant (kobs) shows a hyperbolic dependence on the substrate concentration [S] (26): A hyperbolic fit of the observed rate constants gives the values for the forward (k+ = 181 ± 14 s−1) and reverse (k− = 33 ± 5 s−1) rate constants of the electrogenic partial reaction, as well as for the KD.
The association of f-V122I TTR with the human cardiomyocyte cell line AC16 at 4°C shows a hyperbolic dependence with the f-V122I TTR concentration with an estimated dissociation constant K D=665 nM.
The apparent first-order rate constant of the appearance of the 420 nm band, kobs, shows a hyperbolic dependence on L-Dopa concentratinn in the range 0.08 1 mM (inset of Figure 7).
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