(Thermo Fisher Scientific, Waltham, MA, USA) preceded by a guard column (2 × 2 mm, 5 μm).
Analyses were carried out on an endcapped Superspher RP-18, 125 × 2 mm, 4 µm, column (Merck, Germany), protected by a guard column packed with the same material, and maintained at 40 °C.
Briefly, the anthocyanin extracts were separated through a Zorbax Eclipse XDB-C18 column (Hewlett Packard) (250 × 4.6 mm, 5 μm) preceded by a guard column (12.5 × 4.6 mm) with the same stationary phase, and both columns were maintained at 30 °C.
protected by a guard column (XbridgeTM Phenyl, 10 × 2.1 mm id, 3.5 μm, waters Inc., USA).
The separation of analytes was performed on a Supelcosil LC-ABZ (5 μm, 250 × 4.6 mm) analytical column protected by a guard column (Supelco, Bellefonte, PA, USA).
A μBondapak C18 (5 μM) column (250 × 4.6 mm I.D. 12109949TS) was used; this was preceded by a guard column containing C18/Corasil Bondpak (particle size 37 50 μM).
In our studies, we explored a further reduction of the analysis time by using only a guard column as the simplest and most basic column available.
Separation was performed on a CarboPac PA10 analytical column (250 × 4 mm) with a guard column CarboPac PA10, by an isocratic elution of NaOH 18 mM at 25°C and a flow rate of 1 mL/min.
Released cellooligosaccharides were detected by HPLC using a CarboPac PA100 Carbohydrate Column equipped with a guard column and an ED40 electrochemical detector (all equipment from DIONEX).
Separation was achieved by reverse phase column with an octadecylsilane stationary phase (Merck Lichrospher 100 RP125×4125×4) mm, 5 μm) using a guard column (Merck Lichrospher 100 RP18e (4×4) mm, 5 μm).
