Sentence examples for by a gradient from from inspiring English sources

The eluting solvent started with a 10/90 CH3CN/(water 0.5 % TFA) gradient for 5 min at a flow rate of 2 mL min−1 followed by a gradient from 10/90 to 70/30, v/v, for 25 min.

The 5 µL of each SCX fraction was loaded onto the column, and the mobile phase was held at 100% A (0.1% formic acid) for 20 min, followed by a gradient from 0 to 70% buffer B (0.1% formic acid in 90% acetonitrile) over 85 min with a flow rate ∼500 nL/min.

Peptides were eluted by a gradient from 0.5% formic acid in water to 0.5% formic acid in 50% acetonitrile at a speed of approximately 0.2 µl/min.

Elution was carried out by increasing the NaCl concentration by a gradient from 50 mM to 1 M over the course of 10 column volumes.

Fixed proteins were eluted by a gradient from 0 M to 0.8 M NaCl in 50 mM MES (pH 5.6); 24 fractions (1 mL each) were collected at a flow rate of 1 mL.min-1.

The extract was dissolved in phosphate buffer pH 7.0 for identification by HPLC (Shimadzu SPD-20A system, YMC ODS-A column (4.6 id × 250 mm), MeOH 5 mM phosphate buffer (1% MeOH for 20 min followed by a gradient from 1 to 95% MeOH in 20 min), flow rate 0.3 ml/min, 296 nm.

Dissociation analysis of the PCR products was performed by running a gradient from 60 to 95°C to confirm the presence of a single PCR product.

The purification was achieved by running a gradient from 0 to 50% 1M NaCl in 30mM TRIS, pH 8.0 in 16 CV at 5 ml/min.

Bound proteins were then eluted from the column by running a gradient from 0 to 50% of 500 mM Imidazole, 0.3 M NaCl, 50 mM Na phosphate buffer, pH 8.0 in 12 CV.

Following centrifugation, the supernatant was filtered through a 0.2 μM membrane (Nalgene) and subjected to FPLC ion exchange chromatography (HiPrep 16/10 CM Sepharose, 20 mL column, flow rate 5 mL/min, at 4 °C), washed with 200 mL of buffer A, and eluted by flowing a gradient from 100% buffer A to 100% buffer B (20 mM Na acetate, pH 5.1, 500 mM NaCl, and 1 mM EDTA) over 200 mL.

Peptides were eluted from the cartridge by application of a gradient from 0 to 90% acetonitrile in 0.1% formic acid over 40 min at 100 nl/min, and separated by passing through a C-18 reversed phase column (packed in-house with 5 µm particle size packing material from Column Engineering, Ontario, CA).