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In a second step, samples were eluted by a gradient developed from 5to90%0% of solvent B in solvent A over 15 min.
Peptides were loaded onto a PepMap100 trap column (Thermo Scientific, Dionex Co, CA) and separated on a PepMapRSLC analytical column (75 μm × 150 mm; Thermo Scientific, Dionex Co, CA) by a gradient developed from 5% (v/v) acetonitrile, 0.2% (v/v) formic acid to 80% (v/v) acetonitrile, 0.2% (v/v) formic acid in water at a flow rate of 400 nL/min.
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The concept of cryogenic fracturing is that a sharp thermal gradient developed by applying a cryogenic fluid on a rock surface causes a strong local tensile stress that initiates fractures.
On the other hand, taking steps to reduce oxygen consumption demands could benefit the lung by reducing the pressure gradient developed across the microvasculature.
As a result, a velocity gradient develops along the entry.
As a result a sharp gradient develops near the blood vessel.
A proliferation gradient develops between cell division and expansion at the transition zone.
This gradient develops either through overinflation of the alveolus or a reduction of interstitial pressure.
To capture the size effects of local plastic deformation in the matrix, the strain gradient constitutive theory including the rotation and the stretch gradients developed by Fleck and Hutchinson [Strain gradient plasticity, in: J.W. Hutchinson, T.Y. Wu (Eds)., Advance in Applied Mechanics, vol. 33, Academic Press, New York, 1997, p. 295] is adopted.
Designs based on heat integration rely on the use of steady-state temperature gradients developed in the substrate by the actuation of a single reaction chamber to initiate several reactions at progressively lower temperatures.
The separation of naratriptan hydrochloride and its related products (impurities and degradation products) was obtained by developing a gradient high-performance liquid chromatography method with diode-array detector (HPLC-DAD).
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