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In the case of treatment failure at a given dose level, it was intended to confirm the safety of the corresponding next higher dosage level (+2 Gy) by a further sample of 24 patients for a population of 31 patients on this dose level (Simon, 1989).

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The linkages established however can only be defined as "confirmed linkages" once they have been confirmed in a further sample, preferably by an independent group of investigators [ 49].

However, the MCMC samples for the positive condition in the non-melancholic group did not converge using the criteria of Geweke [ 39] and, thus, a burn-in of 5000 samples (i.e. where the first 5000 samples are discarded, followed by a further 10,000 samples being drawn) for all positive chains across all groups was used.

On each day the first sample was collected immediately upon awakening, followed by a further three samples at 15-minute intervals which collectively comprised the "awakening samples".

Indeterminate results were resolved by testing of a further serum sample obtained at least two weeks later.

Thus, we sought a more equitable sampling distribution by further sampling at most 5 sequences at random for each month in each region.

The three years of monitoring, however, were not sufficient to reveal the total fungal species richness and estimation factors showed that a fungus to plant species ratio of 6 1 may be reached by further sampling efforts.

We fully agree with the reviewer that the primary advance is likely to be brought about by further sampling of diverse archaea that is expected to reveal a greater diversity of artubulins.

Parathyroid hormone (PTH) levels were measured preoperatively before draping, followed by further samples at the time of extirpation and 10 min thereafter.

Species accumulation curves were nearly identical for both approaches and did not reach saturation (Fig. 2, see also supplementary material Fig. S2), indicating that by further sampling additional species would probably have been found.

Finally, our results would be strengthened by replication in further samples.

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