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The mutant lines KK24, MM106 and M25 were previously identified by a forward screen of X-ray induced mutant lines [ 16, 17] of the soybean cultivar 'Bay' [ 18].
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2) A strength of this work is that patronin was identified in a forward screen.
The predicted interactions were further explored between up-regulated miRNAs and a set of "drivers" of CC as defined by a forward genetic screen in mice [ 30].
In addition, Top2b has been identified as the main regulator of ganglion cell axon path finding during zebrafish retinal development by a forward genetic screen (Nevin et al., 2011).
To complement a genome-wide transcriptional survey, we assessed the relative fitness contribution of individual gene products under TMP stress by using a forward genetic screen.
Despite this challenge, some key genes that function in morphogenesis have been identified by standard forward screens and by a variety of elegant modifications of such screens (M etzger and K rasnow 1999; B eitel and K rasnow 2000; S tarz -G aiano and M ontell 2004; Z ohn et al. 2005; M aybeck and R oper 2009; E llertsdóttir et al. 2010; R ochlin et al. 2010; S zabo -R ogers et al. 2010).
TS alleles were generated by way of a forward genetic screen using chemical mutagenesis, as previously described (Kemphues et al. 1988; Encalada et al. 2000; O'Rourke et al. 2011a).
Since then, LIN-35 has been implicated in a number of distinct developmental and cellular processes, which include the characterization of seven lin-35 -synthetic-lethal mutations that were identified by our laboratory through a forward genetic screen (Fay et al. 2002).
Because the Swip assay is a simple, rapid, and highly reproducible method for detecting genetic contributions to endogenous DA signaling, we implemented Swip in a forward genetic screen by using multiple, secondary tests to eliminate nonspecific mutants.
Mutant lines identified by forward screening can be used to develop functional markers for marker-assisted breeding.
The methodical gene-by-gene nature of this approach has several advantages over a forward genetic screen (e.g., in time and labor costs).
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