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Cataracts are a clouding of the lens or its surrounding transparent membrane, characterised by a forward light scatter, and therefore stops it from focusing on the retina [ 1].
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The C-Quant psychometrically measures the retinal straylight as a forward light scattering in a large visual angle of 5 to 10 degrees by the compensation comparison method.
Cells were counted with a fluorescence-activated cell sorting (FACS) Calibur flow cytometer and CellQuest Pro software (BD Biosciences, Mountain View, CA, USA) and single cells were gated away from clumped cells by using forward light scattering on an FL2-width versus FL2-area dot plot.
The morphological profile of a cell can be observed by combining forward light scatter (FSC) and orthogonal or side light scatter (SSC).
The growth of individual cells (as measured by forward light scatter, a proxy for size) began at dawn, and ended two hours before dark, when the cells began to divide (Figure 2A).
First, after lysis of red blood cells (RBCs), the tungsten-halogen based optical system of the MPO channel measures cell size by forward light scatter, and stain intensity by absorbance, thereby counting and differentiating granulocytes, lymphocytes, and monocytes based on their size and MPO content.
Logarithmic amplification of fluorescent intensity on OBA9 (5,000 cells) was determined by forward light scatter intensity.
BST were identified by their size (forward light scatter) and granulosity (side light scatter) as previously described [34].
Dead cells were excluded by forward light scatter or forward light scatter plus propidium iodide.
Dead cells were excluded by forward light scatter and propidium-iodide staining.
By contrast, the OQAS measures the forward light scattering by analyzing the retinal image of a point source of light obtained after focalization of an infrared beam in smaller visual angle within 20 minutes of an arc by the double-pass method.
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