Sentence examples for by a fluorometric method from inspiring English sources

For quantification of DNA levels, 250 300 mg of WAT were homogenized in 3 volumes of PBS, and in the case of rpWAT of 25 day offspring, 60 70 mg in 9 volumes of PBS, using a polytron homogenizer, and then centrifuged at 500 g for 10 min; the supernatant was collected and used for DNA quantification by a fluorometric method that using 3,5 diaminobenzoic acid [29].

Serum retinol levels were determined by a fluorometric method in patients with colorectal cancer or polyps and those with inflammatory bowel disease.

All libraries were quantified by a fluorometric method, using the Qubit dsDNA HS assay kit (Life Technologies, Carlsbad, California, USA), and concentrations were normalized to give equal mitogenome coverage from each individual fish.

After centrifugation at 25,000 g for 20 min, the lysate and supernatant were sonicated for 15 sec and the level of DNA in each fraction was measured by a fluorometric method using DAPI.

CMC of each surfactant was determined by a fluorometric method as described, using 30 μM 1-anilinonaphthalene-8-sulfonic acid (1,8-ANS) (Sigma 10417-5G-F) for each assay, and measured at 25 and 37 °C in 100 mM NH4HCO3, NH4HCO3/1.0 M guanidine, and NH4HCO3/1.0 M guanidine/20% acetonitrile to accurately mimic each digestion condition tested.

Each stage of the impactor was calibrated using a gravimetric or counting method and the fully assembled impactor was evaluated by using a fluorometric method.

Urinary NAG levels were measured following a fluorometric method described by Leadback and Walker [ 9] as well as by Woolen and Walker [ 10] and subsequently modified by Gnanadurai et al [ 11].

The results obtained by the proposed method were compared with those determined by an established HPLC method [ 27] and with a fluorometric method established in our laboratory [ 29].

Here we present a fluorometric method for direct determination of supernatant-free fluorescence spectra generated from fluorescently stained cells in suspension.

The DAN method is a fluorometric method for nitrite quantification.

Glucose was analysed with a fluorometric method [ 17].