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The on-line visualisation showed a fast diffusion of the label into the gap of the hair follicle followed by a fluorescent staining in the gap itself.
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The comparison between such maps showed up- and down-regulation of 84 polypeptide chains, out of a total of 700 spots detected by a fluorescent stain, Sypro Ruby.
The same APTES-modified samples were also tested for binding of complementary DNA sequences and the results were less straightforward due to the necessity of DNA visualization by using a fluorescent stain, YOYO-1.
This registration problem can be solved with the Virtual Brain Explorer (ViBE-Z), which is a software tool that maps cellular gene expression data to a 3D standard larval zebrafish by using a fluorescent stain of cell nuclei for image registration [ 36].
The level of phosphoprotein content on the protein arrays was monitored by probing the arrays with a fluorescent stain (Pro-Q Diamond) that binds to phosphoamino acids [ 18].
BrdU incorporation was detected by fluorescent staining using an anti-BrdU antibody (rat, 1 : 500, Accurate Chemical, Westbury, NY, USA).
The Microbiology department at CMC, Vellore, examines an average of 120 smears per day by fluorescent staining, and is externally accredited by the Central TB Division in Delhi and the supranational reference laboratory at the TB Research Center in Chennai.
DNA synthesis was markedly recognized by the fluorescent staining in the treated compared to non-treated cultures.
Glomeruli can be observed by vital fluorescent staining and laser-scanning confocal microscopy without causing acute or long-term damage to brain tissue.
After 24 h, cells on the surface of GO-chitosan film showed better viability as observed by green fluorescent staining (Figure 8g).
The formation kinetics of the VF, and its viral DNA content in particular, were studied by direct fluorescent staining with the blue fluorescent stain DAPI.
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