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At the three different time periods, wells were washed with PBS and images were taken by a fluorescent scope (Zeiss LSM-510).
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While screening the offspring of these two mating cages we picked a single male from cage #2'with extremely bright eyes (when viewed under a fluorescent scope with a EGFP filter) and mated him with 12 virgin females.
To determine colony size, 10 images were captured per well using AnalySIS getIT software and F-view cattachedtoched to an IX71 inverted fluorescent scope with a 20x objective.
Transgenic animals were maintained by selecting for GFP-positive animals under a fluorescent dissecting scope.
Embryos were incubated at 28°C for 14 20 h and then monitored using a fluorescent dissection scope (SteREO Lumar.V12 Carl Zeiss).
Individual F1 progeny were then picked and transferred to new plates and F2 progeny screened under a fluorescent dissecting scope for gland defects.
GFP scoring was done in the following generation, after bleaching and 24 ± 2 hours of maintenance in M9, by photographing 5 μl of M9 containing live L1 larvae on a glass slide, at a resolution of 1.5 pixel/μm under a fluorescent dissection scope equipped with a digital camera.
Fluorescence images were taken by a fluorescent microscope.
Fluorescence images were taken by a fluorescent microscope (Olympus IX71).
All F3 individuals were photographed under the fluorescent scope.
Four beds stood in a room, dimly lit by a fluorescent tube.
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