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Effects of the decoction on apoptosis were evaluated by (a) fluorescent microscopic examination of apoptosis related morphological changes and (b) DNA fragmentation (c) Caspase 3/7 assay.
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Fig. 5 a Fluorescent microscopic images of anisotropic bacteria absorption at 30 um × 30 um microparticles.
A fluorescent microscopic imaging for the expression of P4H beta is presented (Figure 1B).
Figure 5 shows a fluorescent microscopic analysis of MC. Figure 5A represents cells stimulated with IFN-γ and Figure 5B cells stimulated with IFN-γ and Fas ligation.
We used this system to conduct several operations including a SEM monitored micro house construction by assembling and bonding walls and roof, an optical microscope monitored microscopic insemination by inserting a sperm into an ovum, and a fluorescent microscope monitored DNA surgery cutting out an arbitrary gene from a chromosome.
After exposure, Daphnia were placed on a microscopic slide and we examined the complete Daphnia body under a fluorescent microscope.
Upon the cleavage by 2A protein, the fluorescent protein is released and visible under a fluorescent microscope.
The pP38 was detected by green fluorescent signal and phosphorylated tau as the red fluorescent signal under a fluorescent microscope.
The nuclei were collected, and their purity determined by brightfield microscopic inspection after staining in Trypan Blue solution or by fluorescent microscopic inspection using a wheat germ agglutinin (conjugated to Alexa Fluor® 555) plasma membrane stain in combination with DAPI (4′,6-diamidino-2-phenylindole) nuclear staining.
Further the mechanism of cell death was analysed by fluorescent microscopic analysis and also by scanning electron microscopy.
In addition, the nuclear fragmentation, a hallmark of cellular apoptosis, was clearly exhibited by fluorescent microscopic studies after DAPI staining of untreated and GSH-GAuNPs and LA-GAuNPs (40 80 nm -treated HBL cells (Fig. 8a–d).
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