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Then, cationic carriers were added and fluorescence was monitored by a fluorescent microscope.
Fluorescence was detected by a fluorescent microscope, and its intensity in individual cells was analyzed.
Fluorescence images were taken by a fluorescent microscope.
Fluorescence images were taken by a fluorescent microscope (Olympus IX71).
After 2 days, the cells were stained with Hoechst 33342 solution for 1 h in a 37 °C heated-humidified atmosphere with 5%% CO2, and were then observed by a fluorescent microscope (Olympus IX71, Japan).
Briefly, 30 Synchronized young adult worms of transgenic strain TJ356 daf-16 zls356) IV [2] were transferred to the plates with (50 μM) or without Ot B, and cultured for 12-24 h at 20 °C, then monitored DAF-16::GFP signal by a fluorescent microscope system (Olympus, IX51).
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To remove the excess dye and stain, the nucleus for quantitative analysis, samples were washed with PBS and resuspended in PBS containing DAPI. Green fluorescence and the respective DAPI images were captured by using a fluorescent microscope.
The blastocysts were stained with 5 µg/ml bisbenzimide (Hoechst 33342) to determine the number of nuclei by using a fluorescent microscope.
After examination of 1000 GCs stained by DAPI with a fluorescent microscope, we determined the percentages of apoptotic GCs.
Cells were rinsed with PBS and incubated with 5 µg/ml DAPI for 10 min at room temperature followed by observation under a fluorescent microscope.
Cells were also observed by means of a fluorescent microscope (Leica DMBL; Leica Microsystems GmbH, Wetzlar, Germany) after 24, 48, and 72 hours.
More suggestions(15)
by a fluorescent marker
by a stereoscopic microscope
by a fluorescent siRNA
by a fluorescent staining
by a typical microscope
by a fluorescent plate
by a fluorescent detector
by a fluorescent donkey
by a fluorescent scope
by a conventional microscope
by a fluorescent treponemal
by a fluorescent dye
by a binocular microscope
by a fluorescent molecule
by a fluorescent polarization
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