After centrifugating the homogenates, the supernatants were collected, derivatized with ethylenediamine to detect L-dopa or with diphenylethylenediamine to detect catecholamines, and then analyzed by HPLC using a fluorescent detector.
Next, the MNBDH was oxidized by H2O2 in the presence of DNAzyme for 10 min before HPLC separation, which was monitored by a diode array and a fluorescent detector (λex = 480, λem = 556) and resulted in two peaks at 3.4 and 4.2 min with colorimetric detection and only one peak (Rt = 4.3) when a fluorescence detector was used.
The LOD and LOQ were found to be between 0.054 and 0.16 μg/L and 0.18 and 0.53 μg/L, respectively, using a fluorescent detector.
The UPLC system consisted of a quaternary solvent manager, a sample manager-FTN, a column manager and a fluorescent detector (PDA eλ Detector).
PAHs were detected using a fluorescent detector (excitation wavelength of 260 nm and emission wavelength of 420 nm).
Products of acidic degradation of polymeric proanthocyanidins were separated with the Knauer Smartline chromatograph (Berlin, Germany) equipped with a UV Vis P2800 detector (Knauer, Berlin, Germany) and a fluorescent detector (FD) RF-10AXL (Schimadzu, Tokyo, Japan).
Detection of flavanols and their metabolites was performed using a fluorescent detector with an excitation wavelength of 276 nm and an emission wavelength of 316 nm.
The flow was 0.5 1.0 mL/min from 0 to 12 min and was then set at a constant rate (1.0 mL/min) until the 30th min. The fluorescent intensity was monitored by a fluorescence detector (UltiMate 3000 RS, Dionex Corp., Sunnyvale, CA, USA) with excitation at 320 nm and emission at 512 nm.
The supernatant was analyzed by high performance liquid chromatography (HPLC) with a UV/VIS and fluorescent detector according to Epler et al., 1993 [ 16] with modifications.
The chromatographic analysis was accomplished by means of an Alliance 2695 chromatographic system (Waters, Milford, MA) with an FD 2475 fluorescent detector (Waters, Milford, MA).
