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T-cell immunogenicity was examined longitudinally by a flow cytometry (CD107a, IFNγ, TNFα, IL-2 and/or MIP1β expression) as well as IFNγ ELISPOT.
The cells were then detected by a flow cytometry.
The relative extent of cellular uptake was qualitatively analyzed by a flow cytometry method.
Genome DNA contents (pg/2C) were determined by a flow cytometry analyzer EC800 (Sony Biotechnology Inc., CA, USA).
(D) The percentages of IL-17A+ cells in F4/80+ PEMs were detected by a flow cytometry.
The LSCC-RP9 cells (avian leukosis virus-transformed B-lymphoblastoid cell line from chickens) were used as target cells of PBMC in the peripheral blood for NK cytotoxic activity assay by a flow cytometry using the DiOC/18 membrane dye (Molecular Probes, Eugene, OR) to stain alive LSCC-RP9 cells and PI nuclear dye to stain the dead cells (Kushima et al. 2003).
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The flow cytometry data were analyzed by FLOWJO, a flow cytometry software (Tree Star, Ashland, OR, USA).
Binding of IgG antibody to HSV-2-infected cells was quantified by a flow cytometry-based assay, which is summarized in Fig. S4.
Intravascular and interstitial neutrophils were distinguished by a flow cytometry-based method as described previously [ 33].
Their immunotherapeutic effect was investigated by a flow cytometry-based cytotoxicity assay.
The nonspecific cytotoxic activity of NK cells was quantified by a flow cytometry-based assay using NKTEST® (Orpegen Pharma, Heidelberg, Germany).
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