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NMR and surface plasmon resonance (SPR), among other technologies, show that MPER adopts an α helical conformation partially embedded in the viral membrane, consisting of two independent domains separated by a flexible hinge (Sun et al., 2008; Song et al., 2009).
The two are connected by a flexible hinge region.
These proteins consist of two globular domains with a α/β fold that are connected by a flexible hinge.
The protein kinase domains have a common fold consisting of two lobes linked by a flexible hinge region (see Figure 1A).
The HP1 isoforms share a common structural organization with a chromodomain and a chromoshadow domain in the amino- and carboxy-terminus, respectively, separated by a flexible hinge region.
Tight complex formation requires dimerization of both partners and the correct alignment of the binding surfaces, which is promoted by a flexible hinge domain between repeats 23 and 24 of FLNa.
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Polybodies, with higher avidity for the antigen than single scFv molecules, can be obtained by varying the length of the linker or by connecting via a flexible hinge to an amphipathic helix [ 3, 4].
It consists of 1066 amino acids, has a molecular weight of 117 kDa and, based on its protein structure, can be divided into a head region (residues 1 835) and a tail region (residues 896 1066) connected by a proline-rich region and a flexible hinge (residues 836 895) (10).
Therefore, the PTS1 is now defined as a C-terminal 12-amino-acid sequence, which consists of the C-terminal tripeptide that interacts with the PTS1-binding site in Pex5, a tetrapeptide immediately upstream of this tripeptide, which may interact with the surface of Pex5, and a flexible hinge region of five residues (reviewed by Brocard and Hartig 2006).
The fusion gene was ligated via a flexible hinge tetrapeptide and expressed as a soluble protein in Escherichia coli BL21 (drivenriven by the T7 promoter.
LacI has a C-terminal four-helix bundle tetramerization domain that may act as a flexible hinge.
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